Maintenance of Epigenetic Memory in Cloned Embryos
نویسندگان
چکیده
منابع مشابه
O-7: Improved In Vitro Development of Cloned Bovine Embryos Using S-Adenosylhomocysteine,A Non-Toxic Epigenetic
Background: Development of cloned bovine embryos. Materials and Methods: Oocytes collection,oocyte denudation, oocyte enucleation, nuclear transfer, electrofusion, reconstructed embryo activation, culture of reconstructed and IVF embryo,and treatment with SAH post fusion interval Treatment of reconstructed embryos with TSA for 12 hours after activation, preparation of somatic donor cells, donor...
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Understanding the cellular and molecular mechanisms underlying the formation and maintenance of memories is a central goal of the neuroscience community. It is well regarded that an organism's ability to lastingly adapt its behavior in response to a transient environmental stimulus relies on the central nervous system's capability for structural and functional plasticity. This plasticity is dep...
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We investigated the effects of exposure time and concentration of trichostatin A (TSA) on in vitro development and quality of bovine SCNT embryos. At multiple time points, the relative expression of genes related to pluripotency and reprogramming was analyzed in order to assess the quality of preimplantation embryos cultured in media with TSA using real-time PCR. Development into blastocysts wa...
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Incomplete DNA methylation reprogramming in cloned embryos leads to poor cloning efficiency. Epigenetic modification agents can improve genomic methylation reprogramming and the development of cloned embryos, however, the effect of epigenetic modification agents on gene-specific methylation reprogramming remains poorly studied. Here, we investigated DNA methylation reprogramming of pluripotency...
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(SCNT) embryos of buffaloes. 2. To study gene expression profile of important developmental genes at different stages of SCNT cloned embryo. 3. To study epigenetic reprogramming during early developments of SCNT embryos Materials and Methods: Expression analysis of developmental genes was done in different (ovarian granulose and cumulus and skin fibroblasts) donor cells; in vitro maturing oocyt...
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ژورنال
عنوان ژورنال: Cell Cycle
سال: 2005
ISSN: 1538-4101,1551-4005
DOI: 10.4161/cc.4.6.1743